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Morphology and morphometric diversity of pollen grains of 26 Indian species of Dioscorea L. (Dioscoreaceae)


Pollen morphology of 26 species of Dioscorea collected from different regions of India was examined critically under LM and SEM to explore their unique features. Pollen grains were monad, isopolar, small to medium-sized (11.3–31 µm). Peroblate, oblate, suboblate and oblate spheroidal (P/E=0.41–0.90) pollen shape, mono and bi-sulcate aperture type and psilate, reticulate, rugulose, strito-reticulate, reticulate-rugulose, punctate and striate exine ornamentation were believed to be some of the important characters for differentiation of closely related species in this studied genus. The comprehensive qualitative and quantitative characters of the pollen grains served as reliable features in taxonomical studies. The observed data were analyzed using PCA and hierarchical clustering to obtain a steady relationship and grouping of the observed species. Histograms, pie chart and bar graph were performed to enumerate the distribution of the quantitative data of various attributes (long axis, short equatorial axis, pollen shape and aperture length). Correlation scatter plot along with density graph helped to understand the interrelationship amongst the studied taxa.


Correlation scatter plot, Dioscorea, Hierarchical clustering, PCA, Systematics



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  1. ALZER, F.C., R.S. COUTO, R.C. LOPES, V. GONÇALVESESTEVES AND C.B.F. MENDONCA. 2021. Palynotaxonomy of Neotropical species of Dioscorea L. (Dioscoreaceae). Palynology 45(1): 73–86.
  2. ANONYMOUS. 1952. The Wealth of India. Vol. 3, D–E. CSIR, New Delhi.
  3. ASHA, K.I. AND G.M. NAIR . 2005. Screening of Dioscorea Species for Diosgenin from Southern Western Ghats of India. Indian Journal of Plant Genetic Resources 18(2): 227–230.
  4. AYENSU, E.S. 1973. Dioscoreales, In: Metcalfe C.R. (ed.) Anatomy of the Monocotyledons. Taxon 22(1): 135–152.
  5. BARMAN, P., K.V. BHAT AND R. GEETA. 2018. Phylogenetic analysis of Indian Dioscorea and comparison of secondary metabolite content with sampling across the tree. Genetic Resources Crop Evolution 65: 1003–1012.
  6. BOLICK, M.R. AND S. VOGEL. 1992. Breaking strengths of pollen grain walls. Plant Systematics and Evolution 181: 171–178.
  7. BOLICK, M.R. 1981. Mechanics as an aid to interpreting pollen structure and function. Review of Palaeobotany and Palynology 35 (1): 61–79.
  8. BOZIC, A. AND A. SIBER. 2020. Mechanical design of apertures and the infolding of pollen grain. Proceedings of the National Academy of Sciences of the United States of America 117(43): 26600–26607.
  9. BURKILL, I.H. 1960. The organography and the evolution of Dioscoreaceae, the family of the yams. Botanical Journal of the Linnaean Society 56: 319–412.
  10. CADDICK, L.R., C.A. FURNESS, K.L. STOBART AND P.J. RUDALL. 1998. Microsporogenesis and pollen morphology in Dioscoreales and allied taxa. Grana 37: 321–336.
  11. CARTAXO-PINTO, S., C.B.F. MENDONÇA, R.C. LOPES AND V. GONÇALVES-ESTEVES. 2017. Pollen morphology of species of Cissus (Vitaceae), an evaluation of ornamentation. Palynology 41(3): 359–369.
  12. COUTINHO, A.P., R.A. ALMEIDA D SILVA, D.S. BANDEIRA AND S. ORTIZ. 2012. Pollen morphology in tribe Dicomeae Panero and Funk (Asteraceae). Plant Systematics and Evolution 298: 1851–1865.
  13. COSTA, C.M. AND S. YANG. 2009. Counting pollen grains using readily available, free image processing and analysis software. Annals of Botany 104: 1005–1010
  14. DAJOZ, I., I. TILL-BOTTRAUD AND P.H. GOUYON. 1991. Evolution of pollen morphology. Science 253: 66–68.
  15. DAJOZ, I., A. MIGNOT, C. HOSS AND I. BOTTRAUD. 1995. Pollen Aperture Heteromorphism is not Due to Unreduced Gametophytes. American Journal of Botany 82(1): 104–111.
  16. ERDTMAN, G. 1986. Pollen and Plant Taxonomy Angiosperms, 149. New York: Hafner Publishing Co.
  17. ERDTMAN, G. 1969. Handbook of Palynology: Morphologytaxonomy Ecology: An Introduction to the Study of Pollen Grains and Spores. New York: Hafner Publishing Co. ERDTMAN, G. 1960. The acetolysis method. A revised description. Svensk Botanisk Tidskrift 39: 561–564.
  18. ERDTMAN, G. 1963. Palynology. In: Preston R.D. (ed.) Advances in Botanical Research, 149–208. London-New York: Academic Press.
  19. FAEGRI, K. AND J. IVERSEN. 1964. Textbook of Pollen Analysis. Oxford: Blackwell Scientific Publications.
  20. FURNESS, C.A. AND P.J. RUDALL. 2000. The systematic significance of simultaneous cytokinesis during microsporogenesis in monocotyledons. In: Wilson K.L., Morrison D.A., (eds.) Systematics and evolution of monocots. Proceedings of the Td International Monocot Conference. Melbourne, CSIRO Publishing, 189–193.
  21. GOVAERTS, R., P. WILKIN AND R.M.K. SAUNDERS. 2007. World checklist of Dioscoreales, Yams and their allies. Kew: The board of Trustees of the Royal Botanic Gardens. GREW, N. 1682. The Anatomy of Plants. With an Idea of a Philosophical History of Plants. W. Rawlin, London.
  22. HAMMER, O, D.A.T. HARPER, R. PAUL. 2001. PAST: Paleontological Statistics Software Package for Education and Data Analysis. Palaeontologia Electronica 4(1): 1–9.
  23. HE, Z., C. HONGYAN, G. LI, Z. HONGYAN, Y. GAO, L. ZHANG & S. JIAMING. 2014. Diosgenin inhibits the migration of human breast cancer MDA–MB–231 cells by suppressing Vav2 activity. Phytomedicine 21: 871–876
  24. HESLOP-HARRISON, J. 1976. The physiology of the pollen grain surface. Proceedings of Royal Society London 190: 275–299.
  25. HOOKER, J.D. 1892. Dioscoreaceae. In: Hooker J.D. (ed.) The Flora of British India, Vol. 6. 288–297. London: L. Reeve & Co.
  26. HOQUE, A. 2020. Taxonomic Revision of the family Dioscoreaceae in India. PhD thesis, University of Calcutta, Kolkata, India.
  27. HSU, K.M., J.L. TSAI, M.Y. CHEN, H.M. KU AND S.C. LIU. 2013. Molecular phylogeny of Dioscorea (Dioscoreaceae) in East and Southeast Asia. Blumea 58: 21–27.
  28. HU, S., D.L. DILCHER, M.J. DAVID AND D.W. TAYLOR. 2008. Early steps of angiosperm-pollinator coevolution. Proceedings of the National Academy of Sciences 105(1): 240–245.
  29. HUBER, H. 1998. Dioscoreaceae. In: Kubitzki K. (Ed.) Flowering Plants Monocotyledons. The Families and Genera of Vascular Plants, Vol. 3. 216–235. Berlin, Heidelberg: Springer.
  30. HUBER, H. 1977. The Treatment of the Monocotyledons in an Evolutionary System of Classification. In: Kubitzki K. (Ed.) Flowering Plants. Plant Systematics and Evolution. Suppl 1: 258–298.
  31. KAPADIA, Z.J. AND F.W. GOULD. 1964. Biosystematic studies in the Bouteloua curtipendula complex. III. Pollen size as related to chromosome numbers. American Journal of Botany 51: 166–172.
  32. KNUTH, R. 1924. Dioscoreaceae. In: Engler H.G.A., Leipzig H.Y. (eds.) Das Pflanzenreich. 1–387.
  33. KUMAR, S., G. DAS, H. SHIN AND J.K. PATRA. 2017. Dioscorea spp. (A Wild Edible Tuber), A Study on its Ethnopharmacological Potential and Traditional Use by the Local People of Similipal Biosphere Reserve, India. Frontiers in Pharmacology 8 (52): 1–17.
  34. KUPRIANOVA, LA. 1967. Apertures of pollen grains and their evolution in Angiosperms. Review of Paleobotany and Playnology 3: 73–80.
  35. LAU, T.C., X. LU, R.T. KOIDE AND A.G. STEPHENSON. 1995. Effects of soil fertility and mycorrhizal infection on pollen production and pollen grain size of Cucurbita pepo (Cucurbitaceae). Plant Cell Environment 18: 169–177.
  36. LECHOWICZ, K., D. WROŃSKA-PILAREK, J. BOCIANOWSKI AND T. MALIŃSKI. 2020. Pollen morphology of Polish species from the genus Rubus L. (Rosaceae) and its systematic importance. PLoS ONE 15(5): 1–23.
  37. MABBERLEY, D.J. 2017. Mabberley’s Plant-Book-A Portable Dictionary of the Vascular Plants. 4th edition. 231–232. Cambridge: Cambridge University Press.
  38. MATHEW, P.M. AND A. MATHEW. 1980. Induced polyploidy in Tagetus erecta. Cytologia 45: 803–807.
  39. MIGNOT, A., C. HOSS, I. DAJOZ, C. LEURET, J.P. HENRY, J.M. DREUILLAUX, E. HEBERLE-BORS AND I. TILLBOTTRAUD. 1994. Pollen aperture polymorphism in the Angiosperms: importance, possible causes and consequences. Acta Botanica Gallica 141(2): 109–122.
  40. MI-MI, L., Y. QIN-QIN, X.Q. SUN, Y.M. ZHAO, Z. YI-FENG AND H. YUE-YU. 2014. A preliminary study on pollination biology of three species in Dioscorea (Dioscoreaceae). Life Science Journal 11(2): 436–444.
  41. MOSYAKIN, S.L. AND Z.M. TSYMBALYUK. 2017. Pollen morphology of the tribe Hemimerideae: Possible evidence of ancestral pollen types and parallel evolution in the basal most clade of Scrophulariaceae s. str. Willdenowia 47: 15–27.
  42. ONG, E.T. AND A.N. RAO. 1973. Pollen dimorphism in certain angiosperms. Journal of Palynology 9: 142–151.
  43. PAUL, P. AND M. CHOWDHURY. 2020. Pollen morphology of selected Indian species from subfamily Polygonoidae (Polygonaceae). Biologia 75(3): 1–13.
  44. PAYNE, W.W. 1972. Observations of harmomegathy in pollen of Anthophyta. Grana 12: 92–98.
  45. PAYNE, W.W. 1981. Structure and function in angiosperm pollen wall evolution. Review of Palaeobotany and Palynology 35: 39–59.
  46. PERSSON, V., S. KANPPS AND S. BLACKMORE. 1996. Pollen morphology and systematic of tribe Juanulloeae a. T. Hunziker (Solanaceae). Review of Palaeobotany and Palynology 83: 1–30.
  47. PIGNOTTI, L. AND L.M. MARIOTTI. 2004. Micromorphology of Scirpus (Cyperaceae) and related genera in south-west Europe. Botanical Journal of the Linnaean Society 145: 45–58.
  48. PRAIN, D. AND I.H. BURKILL. 1936. An account of the Genus Dioscorea in the East, Part I. The species which twine to the left. Annals of Royal Botanic Garden (Calcutta) 14(2): 1–210.
  49. PRAIN, D. AND I.H. BURKILL. 1938. An account of the Genus Dioscorea in the East, Part II. The species which twine to the right. Annals of Royal Botanic Garden (Calcutta) 14: 211–528.
  50. PUNT, W., P.P. HOEN, S. BLACKMORE, S. NILSSON AND A. LE THOMAS. 2007. Glossary of pollen and spore terminology. Review of Paleobotany and Palynology 143: 1–81.
  51. RANJAN, V. 2018. Herbaria: a systematic repository of plant specimens for research. In: Maity D., (ed.)Taxonomy: Theory and Practice. Procedings of the first international workshop under Taxonomy Training Centre, AICOPTAX, MoEF & CC, Govt. of India. 96–118. Ruby das, Serampore Hooghly.
  52. LUZ, C.F.P.D., L.O. BARBUGLIO-SANTIAGO, A.R.G. SIMÕES, J.H.D. SILVA, V.L.D. SANTOS, M. KIRIZAWA. 2020. Pollen morphology of Dioscorea (Dioscoreaceae) from the Atlantic Forest in southeast Brazil (São Paulo) with a contribution to the systematics of Neotropical species. Grana 59(4): 239–257.
  53. ROYTCHEV, V. 2009. Application of Cluster Analysis and Principal Component Analysis in Palynobiometric studies of seedless cultivars and hybrid vine forms (Vitis Vinifera L.). Bulgarian Journal of Agricultural Science 15 (1): 31–40.
  54. SCHOLS, P., C.A. FURNESS, P. WILKIN, S. HUYSMANS AND E. SMETS. 2001. Morphology of pollen and orbicules in some Dioscorea species and its systematic implications. Botanical Journal of the Linnaean Society 136: 295–311.
  55. SCHOLS, P., C.A. FURNESS, P. WILKIN, E. SMETS, V. CIELEN AND S. HUYSMANS. 2003. Pollen morphology of Dioscorea (Dioscoreaceae) and its relation to systematics. Botanical Journal of the Linnaean Society 143: 375–390.
  56. SELLING, O.H. 1947. Studies in Hawaiian pollen statics–Part– II. The pollens of the Hawaiian Phanerogams. B.P. Bishop Museum, Special Publication 38: 1–430.
  57. SHAIJU, P.N. 2012. PoIIen Morphology of the Genus Thottea. Phytomorphology 62 (3 & 4): 125–135.
  58. SHEIKH N. AND Y. KUMAR. 2017. Morphology of flower, pollen and orbicules of Meghalayan Dioscorea L. (Dioscoreaceae), North-East India: A pivotal taxon in the evolution of monocot. Asian Journal of Biological Science 12(2): 223–232.
  59. SHEIKH, N. AND Y. KUMAR. 2018. Palynological Implication to the Systematic of the Genus Dioscorea in Meghalaya, North East India. Indian Journal of Pure & Applied Biosciences 6 (1): 94–100.
  60. SHEIKH, N., Y. KUMAR, L. JERI AND N.A. BHAT. 2017. Ethnobotanical Uses and Survey of Dioscorea Species of North East India, Its Conservation and Sustainable Utilization. International Journal of Current Research in Biosciences and Plant Biology 4 (12): 117–124.
  61. SNEATH, P.H.A. AND R.R. SOKAL. 1973. Numerical Taxonomy. San Fran-cisco: W. H. Freeman and Company.
  62. STEPHENSON, A.G.C., C. ERICKSON, T.C. LAU, M.R. QUESADA AND J.A. WINSOR. 1994. Effects of growing conditions on the male gametophyte. In: Stephenson A.G., T-h Kao (eds.) Pollen pistil interactions and pollen tube growth, 220–229. American Society of Plant Physiologists, Rockville, Md.
  63. SU, P. 1987. Pollen morphology of Dioscorea in China. Acta Phytotaxonomica Sinica 25: 357–365.
  64. TAKHTAJAN, A. 1997. Diversity and Classification of Flowering Plants Dioscoreaceae. 524. New York: Columbia University Press.
  65. TING, C. AND M.G. GILBERT. 2000. Dioscoreaceae. In: Wu Z.Y., Raven P.H. (eds.) Flora of China. Vol. 24: 276–296. Beijing: Science Press and St. Louis: Missouri Botanical Garden.
  66. VAN DER HAM, R.W.J.M., W.L.A. HETTERSCHEID AND B.J. VAN HEUVEN. 1998. Notes on the genus Amorphophallus (Araceae)–8: Pollen morphology of Amorphophallus and Pseudodracontium. Review of Palaeobotany and Palynology 103: 95–141.
  67. VEZEY, E.L., V.P. SHAH AND J.J. SKVARLA. 1992. A numerical approach to pollen sculpture terminology. Plant Systematics and Evolution 181: 245–254.
  68. WALKER, J.W. 1974. Aperture evolution in the pollen of primitive Angiosperms. American Journal of Botany 61: 1112–1137.
  69. WALKER, J.W. AND J.A. DOYLE. 1975. The Bases of Angiosperm Phylogeny: Palynology. Annals of the Missouri Botanical Garden 62: 664–723.
  70. WARD, J.H. 1963. Hierarchical clustering to optimize an objective function. Journal of the American Statistical Association 58 (301): 236–244.
  71. WILKIN, P., P. SCHOLS, M.W. CHASE, K. CHAYAMARIT, C.A. FURNESS, S. HUYSMANS, F. RAKOTONASOLO, E. SMETS AND C. THAPYAI. 2005. A plastid gene phylogeny of the yam genus, Dioscorea: roots, fruits and Madagascar. Systematic Botany 30: 736–749.
  72. WODEHOUSE, R.P. 1935. Pollen Grains. New York: Mc. Grew Hill.
  73. XIFREDA, C.C. 2000. Evaluation of pollen and vegetative characters in the systematics of South American species of Dioscorea (Dioscoreaceae). In: Wilson K.L., Morrison D.A., (eds.) Proceedings of the 2nd International Monocot Conference, Systematics and evolution, Melbourne, CSIRO, 488–496.
  74. ZHANG, M.Y., L. LU, A.H. WORTLEY, H. WANG D.Z. LI AND S. BLACKMORE. 2017. Evolution of Angiosperm Pollen: 4. Basal Eudicots. Annals of the Missouri Botanical Garden 102: 141–182.