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Volume 64, Issue 1, July 2022

Articles

Pollen Morphology of some Taxa of Genus Gentiana L. (Gentianaceae) from Western Himalaya, India

https://doi.org/10.20324/nelumbo/v64/2022/166479

Published 2022-08-02

  • Abhishek Kumar
    • ,
  • Mohd. Shabir
    • ,
  • Eja Gloch
    • ,
  • Prabhawati Tiwari
    • ,
  • R. D. Gaur

Abhishek Kumar
Deparment of Botany and Biodiversity Research Centre, Kargil Campus, University of Ladakh, Saliskote Kargil - 194105, UT-Ladakh

Mohd. Shabir
Deparment of Botany and Biodiversity Research Centre, Kargil Campus, University of Ladakh, Saliskote Kargil - 194105, UT-Ladakh

Eja Gloch
Department of Botany & Microbiology, HNB Garhwal University, Srinagar (Garhwal) - 246174, Uttarakhand

Prabhawati Tiwari
Department of Botany & Microbiology, HNB Garhwal University, Srinagar (Garhwal) - 246174, Uttarakhand

R. D. Gaur
Department of Botany & Microbiology, HNB Garhwal University, Srinagar (Garhwal) - 246174, Uttarakhand

Abstract

The present study deals with the pollen morphology of 15 taxa of the genus Gentiana L. (Gentianaceae). Polleniferous material was collected from different locations of the Western Himalaya (India). Pollen micro-morphological characters were investigated using light microscopy (LM) and scanning electron microscopy (SEM). All pollen grains were monad, isopolar and radially symmetrical, medium to minutae in size, sub-prolate to prolate in pollen shape class, 3-zonocolpate to 3-zonocolporate, with usually long tapering colpi. In polar view pollen grains were circular to triangular in outline with obtuse end, while elliptic or occasionally sub-circular in equatorial view. Exine was usually thick, tectate or semitectate. The morphology of the pollen grains exhibited significant variation in exine ornamentation viz. striate, striato-reticulate and reticulate, which is useful to evaluate species delimitations. The study revealed that the pollen characteristics appear to be informative and useful atinter- and intra-specific levels.

Keywords

Aperture, Exine Ornamentation, Gentiana, Pollen Morphology, SEM.

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References

  1. CHASSOT, P., S. NEMOMISSA, Y.M. YUAN AND P. KUPFER 2001. High paraphyly of Swertia L. (Gentianaceae) in the Gentianella-lineage as revealed by nuclear and chloroplast DNA sequence variation. Plant Systematics and Evolution, 229:1–21.
  2. ELIAS, T. S. AND A. ROBYNS 1975. Gentianaceae. In: WOODSON, R. E. AND R.W. SCHERY (Eds.), Flora of Panama, Part 8. Annals of the Missouri Botanical Garden, 62: 61–101.
  3. ERDTMAN, G. 1952. Pollen Morphology and Plant Taxonomy: Angiosperms (An introduction to Palynology I). Almqvist and Wiksell. Stockholm.
  4. FAVRE, A., S.J. PRINGLE, J. HEACKENHAUER, E. KOZUHAROVA, Q. GAO, M.E. LEMMON, R.A. LEMMON, H. SUN, N. TKACH, S. GEBAUER, S.S. SUN AND C.P. FU 2020. Phylogenetic relationships and sectional delineation within Gentiana (Gentianaceae). Taxon, 69(6): 1221–1238. https://doi.org/10.1002/tax.12405
  5. FALK, R.H. 1980. Preparation of plant tissues for SEM. Scanning Electron Microscopy. 1980/ⅱ: 79–88.
  6. GILG, E. 1895. Gentianaceae. In: A. ENGLER AND K. PRANTL (Eds.), Die Naturlichen Pflanzen Familien, Vol. 4(2). Verlag von Wilhelm Engelmann, Leipzig. pp. 50–108.
  7. GUISON, A. AND W. THULLER 2005. Predicting species distributions: offering more than simple habitat models. Ecology Letters, 8(9): 993–1009.
  8. GUPTA, S., A. MUKHERJEE AND M. MONDAL 2012. A census of Gentiana L. in India, systematics of flowering plants. In: Maiti, G. and S. K. Mukherjee (eds.), Multidisciplinary Approaches in Angiosperm Systamatics. University of Kalyani, Kalyani, West Bengal, India. Vol. 1: 53–58.
  9. HO, T. N. AND S. W. LIU 1990. The infrageneric classification of Gentiana (Gentianaceae). Bulletin of the British Museum Botany, 20(2): 169–192.
  10. HO, T.N. AND S. LIU 2001. A Worldwide Monograph of Gentiana. Science Press, Beijing, China.
  11. JONSSON, L. 1973. Pollen morphology in African species of Swertia L. (Gentianaceae). Grana Palynology, 13 (2): 119–128.https://doi.org/10.1080/00173137309429887
  12. KLACKENBERG, J. 1985. A re-evaluation of the genus Exacum (Gentianaceae) in Ceylon. Nordic Journal of Botany, 3(3): 355–370. http://dx.doi.org/10.1111/j.1756-1051.1983.tb01951.x
  13. KOHLER, A. 1905. Der Systematische Wert der Pollen beschaffenkeitbei den Gentianaceen. Mitteilungenaus dem¹Botanischen Garten und Museum der Universitat Zurich, 25:1–73.
  14. KOZUHAROVA, E. AND E. BOZILOVA 2001. Pollen morphology of some Gentiana species (Gentianaceae) presented in Bulgarian flora. Annuire de l`Universite de Sofia "st K. Ohridski" Faculte de Biologie Livre 2 Botanique. 93, 83–98.
  15. LAINZ, M. 1976. Aportaciones al conocimiento de la flora Cantabro-Astur, XI. Bol. Inst. Estud. Asturianos 22: 3–44.
  16. LINNAEUS, C. 1753. Species Plantarum, Vol. 1. Holmiae, Sweden, p. 227. MABBERLEY, D.J. 2008. The Plant Book: A Portable Dictionary of Plants, their Classification and Uses. 3: 354. Cambridge University Press. Cambridge.
  17. MAITY, D. 2014. A new species of Gentiana L. (Gentianaceae) from Sikkim Himalaya. Edinburgh Journal of Botany, 71(3): 289-296. http://dx.doi.org/10.1017/s096042861400016x
  18. NAIR, P.K.K. 1965. Trends in the morphological evolution of pollen and spores. Journal of Indian Botanical Society, 44: 468–478.
  19. NILSSON, S. 1967a. Pollen morphological studies in the Gentianaceae- Gentianinae. Grana Palynologica, 7: 46–145.
  20. NILSSON, S. 1967b. Notes on pollen morphological variation in Gentianaceae- Gentianinae. Pollen Spores, 9: 49–58.
  21. NILSSON S. 1968. Pollen morphology in the genus Macrocarpaea (Gentianaceae) and its taxonomical signiï¬cance. Svensk Botanisk Tidskrift, 62: 338–364.
  22. NILSSON, S. 1970. Pollen morphological contributions to the taxonomy of Lisianthus L. s. lat. (Gentianaceae). Svensk Botanisk Tidskrift, 64: 1–43.
  23. NILSSON, S. AND J.J. SKVARLA 1969. Pollen morphology of saprophytic taxa in the Gentianaceae. Annals of the Missouri Botanical Garden, 56: 420–438.
  24. NILSSON, S., M. HELLBOM AND W. SMOLENSKI 2002. A reappraisal of the significance of pollen in classifications of the Gentianaceae. Grana, 41(2): 90–106. https://doi.org/10.1080/001731302760156891
  25. PAMELA, S. S. AND E. S. DOUGLAS 2004. The origin and diversification of angiosperms. American Journal of Botany, 91(10): 1614–1626. https://doi.org/10.3732/ajb.91.10.1614
  26. PANICKER, K.T.C. 2004. Studies in the pollen morphology of Mimosaeae monads, tetrad and octad taxa. Journal of Palynology, 40: 9–21.
  27. PERVEEN, A. AND M. QAISER 2007. Pollen Flora of Pakistan-Malvaceae-Grewioideae-LII. Pakistan Journal of Botany. 39: 1–10.
  28. POWO (Plants of the World Online), 2021. Gentiana Tourn. ex L. Board of Trustees of the Royal Botanic Gardens, Kew. Retrieved on December 21, 2021 from https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:30001281-2.
  29. PUNT, W. AND W. NIENHUIS 1976. Gentianaceae. Review of Palaeobotany and Palynology, 21: 89–123.
  30. PUNT, W., S. BLACKMORE, S. NILSSON AND A.L.E. THOMAS 2007. Glossary of pollen and spore terminology. Review of Paleobotany and Palynology, 143: 1–81.
  31. RAO, K.S. AND C.C. CHINNAPPA 1983a. Pericolporate pollen in Gentianaceae. Canadian Journal of Botany, 61(1): 174–178.
  32. RAO, K.S. AND C.C. CHINNAPPA 1983b. Studies in Gentianaceae, Microsporangium and Pollen. Canadian Journal of Botany, 61(1): 324–336.
  33. SHABIR, M. 2019. Monographic and Phytochemical Studies on the Genus Gentiana L. in India. Ph.D. thesis. HNB Garhwal University, Srinagar Garhwal, India.
  34. SHABIR, M., P. AGNIHOTRI, D. HUSAIN, J.K. TIWARI AND T. HUSAIN 2017. On the current status of the genus Gentiana L. (Gentianaceae) in India. Pleione, 11(1): 16–24.
  35. SOLTIS, P.S. AND D.E. SOLTIS 2004. The origin and diversification of angiosperms. American Journal of Botany, 91(10): 1614–1626.
  36. STRUWE, L., J.W. KADEREIT, J. KLACKENBERG, S. NILSSON, M. THIV, K.B. VON HAGEN AND V.A. ALBERT 2002. Systematics, character evolution, and biogeography of Gentianaceae, including a new tribal and sub tribal classiï¬cation. In: L. STRUWE AND V.A. ALBERT (Eds.), Gentianaceae: Systematics and Natural History. Cambridge University Press, Cambridge, pp. 21–309.
  37. WHITMORE, T.C. 1998. An Introduction to Tropical Rain forests. Oxford University Oxford, U.K, Press. pp. 1–246.
  38. WODEHOUSE, R.P. 1935. Pollen Grains. McGraw Hill, New York.