Skip to main navigation menu Skip to main content Skip to site footer

Antimicrobial and in vitro Efficacy of Green Silver Nanoparticles in Tissue Culture of Alhagi maurorum


Biologically synthesized silver nanoparticles (SNPs) from Alhagi maurorum used as disinfectant, and in callogenesis and organogenesis. Green SNPs 125 and 150 mg/l were the best for explants sterilization. 2.0 mg/l – 6.0 mg/l SNPs when added in MS medium [(BAP (3.0mg/l) + NAA (0.2mg/l) + Kin (1.0 mg/l) + ascorbic acid 50.0 mg/l and 25 mg/l each of citric acid, arginine and adenine sulphate)] showed better calologenesis and organogenesis. Higher concentrations of SNPs resulted in decline of survival percentage, callus initiation and growth of cultured tissues.


Alhagi mMaurorum, Callogenesis, Decontaminating Agent, Green Silver Nanoparticles.



Download data is not yet available.


  1. ABDI, G.H., H. SALEHI AND M. KHOSHKHUI 2008. Nano silver: A novel nanomaterial for removal of bacterial contaminants in valerian (Alteriana officinalis L.) tissue culture. Acta Physiol. Plant 30: 709–714.
  2. AGARWAL, T., A.K. GUPTA, A.K. PATEL AND N.S. SHEKHAWAT 2015. Micropropagation and validation of genetic homogeneity of Alhagi maurorum using SCoT, ISSR and RAPD markers. PCTOC 120: 313–323.
  3. AGHDAEI, M., H. SALEHI AND M.K. SARMAST 2012. Effects of silver nanoparticles on Tecomella undulata (Roxb·) Seem, micropropagation. Adv Hortic Sci 21-24.
  4. AL-DOURI, N. A., AND L.Y. AL-ESSA 2010. A survey of plants used in Iraqi traditional medicine. Jordan J. Pharm. Sci .3(2): 100-108.
  5. AMANI, A.S., D.J. MAITLAND AND G.A. SOLIMAN 2006. Antiulcerogenic activity of Alhagi maurorum. Pharm. Biol. 44(4): 292–296.
  6. ARRUDA, S. C., A. L. D. SILVA, R. M. GALAZZI, R. A. AZEVEDO AND M. A. Z. ARRUDA 2015. Nanoparticles applied to plant science: a review. Talanta 131: 693-705.
  7. ATTA, A.H. AND K. ABO EI–SOOUD 2004. The antinociceptive effect of some Egyptian medicinal plants extracts. J. Ethnopharmacol 95 (2-3): 235-238.
  8. ATTTA, A.H. AND S.M. MOUNEIR 2004. Anti–diarrhoeal activity of some Egyptian medicinal plant extracts. J. Ethnopharmacol 92 (2-3): 303–309.
  9. BADSHAH, L. AND F. HUSSAIN 2011. People preferences and use of local medicinal flora in district Tank, Pak. J. Med. Plants Res. 5 (1): 22–29.
  10. BOLUS, L. 1983. Medicinal Plants of North Africa. Cairo, Egypt. Reference Publications In, 368, 96-106.
  11. HARRIS, A. T., AND R. BALI 2008. On the formation and extent of uptake of silver nanoparticles by live plants. J. Nano Res . 10(4): 691-695.
  12. HASSANEIN, A.M. AND A.M.A. MAZEN 2001. Adventitious bud formation in Alhagi graecorum. PCTOC 65: 31–35.
  13. HEGAZY, H. S., G.H. RABIE, L.D. SHAABAN AND D.S. RAIE 2014. Extracellular synthesis of silver nanoparticles by callus of Medicago sativa. Life Sci. 11(10): 1211-1214.
  14. HENDAWEY, M.H., R.E.A.E.FADL AND T.A. SALAH EL-DIN 2015. Biochemical role of some nanoparticles in the production of active constituents in Stevia rebaudiana callus. Life Sci. 12: 7.
  15. HONARI, M., H. ASKARI AND KHOSROWCHAHLI 2014. Use of desirability function method in optimization of regeneration and callus induction of Alhagi camelorum. AJPS 5: 268–274.
  16. KAWASE, K. AND I. KANNO 1983. Soils of China: their use and improvement, nature of fertility and soil properties, genesis, classification and distribution. Hakuyusha. Tokyo 854–888.
  17. KHAN, F. M. 2009. Ethno-veterinary medicinal usage of flora of Greater Cholistan desert (Pakistan). Pak. Vet. J. 29 (2).
  18. KOUCHMESHKY, A., S.B. JAMEIE G. AMIN AND S.A. ZIAI 2012. Investigation of ACE inhibitory effects of medicinal plants used in traditional Persian medicine for treatment of hypertension: screening study. Thrita Stud. J. Med. Sci. 1(1): 13-23.
  19. KOUHI S.M.M., M. LAHOUTI, A. GANJEALI, M.H. ENTEZARI 2015. Comparative effects of ZnO nanoparticles, ZnO bulk particles, and Zn2+ on Brassica napus after long-term exposure: changes in growth, biochemical compounds, antioxidant enzyme activities, and Zn bioaccumulation. Water Air Soil Pollution journal 226:364.
  20. LANDFORD, N.J. AND R.E. FERNER, R 1999. Toxicity of Mercury (Review). J. Human Hypertension 13: 651–656.
  21. LONGER, D.E. AND Y. DEGAGO 1996. Field weathering potential of normal and hard seeded soybean genotypes. Seed Sci. Tech. 24: 273-290.
  22. MAHNA, N., S.Z. VAHED AND S. KHANI 2013. Plant in vitro culture goes nano: nanosilver-mediated decontamination of ex vitro explants. J. Nanomed Nanotechol 4(2):1000161.
  23. MALABADI, R. B., R.K. CHALANNAVAR, N.T. METI, G.S. MULGUND, K. NATARAJA AND S.V. KUMAR 2012. Synthesis of antimicrobial silver nanoparticles by callus cultures and in vitro derived plants of Catharanthus roseus. Research in Biotechnology 3(5): 26-38.
  24. MARWAT, S.K., M.A. KHAN, M. AHMAD, M. ZAFAR, AND F. REHMAN, 2008. Ethnophytomedicines for treatment of various diseases in D.I. Khan District. Sarhad J. Agric. 24 (2): 306 - 316.
  25. MILLER, G.W., I.J. HUANG, G.W. WELKIE, AND J.C. PUSHMIK, 1995. Function of iron in plants with special emphasis on chloroplasts and photosynthetic activity. In: Abadia, J., (Ed.), Iron nutrition in soils and Plants. Kluwer Academic Publishers, Dordecht. 19 - 28.
  26. MOHAMED-YASSEEN, Y., S.A. BARRINGER, W.E. SPLITTSTOESSER, W AND S. COSTANZA 1994. The role of seed coats in seed viability. The Botanical Review 60(4): 426-439.
  27. MUDE, N., A. INGLE, A. GADE, AND M. RAI 2009. Synthesis of silver nanoparticles using callus extract of Carica papaya–a first report. Plant Biochem. Biot. 18(1): 83-86.
  28. MURASHIGE, T., AND F. SKOOG 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant 15 (3): 473-497.
  29. NABEEL K.A. 2011. Using Silver Nano- Particles to Increase Efficiency of Sterile Solution for in vitro Techniques. Iraqi J. of Cancer and Med. Genetics 4(1): 48–51.
  30. NABIKHAN, A., K. KANDASAMY, A. RAJ AND N.M. ALIKUNHI 2010. Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids and surfaces B: Biointerfaces 79(2): 488-493.
  31. RAHMAN, S. A., S.A. ABD-ELLATIF, S.F. DERAZ AND A.A. KHALIL 2011. Antibacterial activity of some wild medicinal plants collected from western Mediterranean coast, Egypt: Natural alternatives for infectious disease treatment. Afr. J. Biotechnol 10(52): 10733-10743.
  32. RAMTEKE, K.H., S.A. JOSHI AND S.N. DHOLE 2012. Solid Lipid Nanoparticle: A Review. IOSR J. Pharm 2: 34–44.
  33. RAVEENDRAN, P., J. FU AND S.L. WALLEN 2003. Completely “green†synthesis and stabilization of metal nanoparticles. J. Am. Chem. Soc. 125(46): 13940-13941.
  34. SAFAVI, K. 2012. Evaluation of using nanomaterial in tissue culture media and biological activity. In Second Int. Conf. on Ecological, Environmental and Biological Sciences (EEBS’2012), Bali, Indonesia (pp. 13-14).
  35. SAMEJO, M. Q., S. MEMON, M.I. BHANGER ANDK.M. KHAN 2012. Chemical composition of essential oils from Alhagi maurorum. Chem. Nat. Compd. 48(5): 898-900.
  36. SAXENA, A., R.M. TRIPATHI AND R.P. SINGH 2010. Biological synthesis of silver nanoparticles by using onion (Allium cepa) extract and their antibacterial activity. Dig J Nanomater Bios. 5(2): 427-432.
  37. SHAKER, E., H. MAHMOUD, S. MNAA 2010. Antiinflammatory and anti-ulcer activity of the extract from Alhagi maurorum (camelthorn). Food Chem. Toxicol. 48(10): 2785-2790.
  38. SHARMA, S., N. RANJARIA, B. VARGTRESE, S. KESHAVKANT AND S.C.NAITHANI, S.C. 2008. Effect of hot-water treatment on seed germination of some fast growing tropical tree species. J. Trop. For. Sci., 2008 24: (Ill & lV).
  39. SHEIKHBAGLU, R., M. SEDGHI, H. SALEHIAN AND S. RAHIMZADEH 2014. Spraying effect of maternal plants with nano-iron oxide on germination indices and electrical conductivity of produced soybean seeds. Int. J Biosci 5 (11): 22 - 27.
  40. TORRESDEY, J.L.G., J.G. PARSONS, E. GOMEZ, J. PERALTA-VIDEA, H.E. TROIANI, P. SANTIAGO AND M.J. YACAMAN 2002. Formation and growth of Au nanoparticles inside live Alfalfa plants. Nano. lett. 2 (4): 397– 401.
  41. UPHOF, J. C. T. 1959. Dictionary of economic plants. Dictionary of economic plants.
  42. VARSHNEY, K. AND A. K. SINGH 2008. Inventory of some ethnomedicinal plant species used by rural people of Etah district, UP, India. Plant Arch. 8(2): 757–759.
  43. WHO (2019) Global report on traditional and complementary medicine.
  44. ZOU, G.A., S. MANSUR, S., S.C. HU, H.A. AISA AND K.M. SHAKHIDOYATOV 2012. Pyrrole alkaloids from Alhagi sparsifolia. Chem. nat. prod. 48(4): 635–637.